MOLECULAR BIOSENSORS CAPABLE OF SIGNAL AMPLIFICATION

摘要

The present invention provides molecular biosensors capable of signal amplification, and methods of using the molecular biosensors to detect the presence of a target molecule.

主权项

1. A molecular biosensor comprising three constructs, the constructs comprising:
R1—R2—R3; R4—R5—R6; and at least one R7—R8—R9;  (II)wherein:
R1 is an epitope-binding agent that binds to a first epitope on a target molecule; R2 is a flexible linker attaching R1 to R3; R3 and R6 are a first pair of nucleotide sequences that are complementary to two distinct regions on R8; R5 is a flexible linker attaching R4 to R6; R4 is an epitope-binding agent that binds to a second epitope on a target molecule; R8 is a nucleotide construct comprising a first region that is complementary to R3 and a second region that is complementary to R6, such that when R3 and R6 associate with R8, an endonuclease restriction site is reconstituted; R7 is a signaling molecule; and R9 is a solid support.