Systems and Methods for Diagnosing a Fluidics System and Determining Data Processing Settings for a Flow Cytometer

摘要

The present set of embodiments relates to systems and methods for diagnosing a fluidics system and determining data processing settings for a flow cytometer. Systems and methods for diagnosing a fluidics system require accurate measurement and interpretation of fluctuations within the fluid delivery system. Systems and methods for determining data processing settings require an accurate measurement of peak times among various channels and being able to adjust time delay settings wherein peak time is the measurement of time elapsed from the beginning of the data collection time window to the highest peak in the window.

主权项

1. A method for determining data processing settings for a flow cytometer comprising:
passing a set of calibration particles through a flow cell; illuminating each of the set of calibration particles passing through the flow cell with at least two light beams wherein each light beam is associated with a channel; collecting light emitted from each of the set of calibration particles using a detector associated with each channel; recording data from each detector; setting a trigger channel to initiate a transfer of data from a first data collection time window associated with the trigger channel when a data signal threshold for the trigger channel is exceeded; setting a second channel to transfer data from a second data collection time window associated with the second channel when the data signal threshold for the trigger channel is exceeded, and wherein the start of the second data collection time window is based on a spatial path between the trigger channel and the second channel; recording data from the first data collection time window to a data store each time the data signal threshold is exceeded; recording data from the second data collection time window to the data store each time the data signal threshold for the trigger channel is exceeded; analyzing a distribution of data intensity peak times within the second data collection time window; and calculating a time delay based on the distribution of data intensity peak times in the second data collection time window to position a data signal in the second channel in the second data collection time window.