发明名称 |
NUCLEOTIDE-SPECIFIC RECOGNITION SEQUENCES FOR DESIGNER TAL EFFECTORS |
摘要 |
The invention relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in an animal cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that includes a deoxyribonucleic acid (DNA) binding polypeptide having a N-terminal capping region, a DNA binding domain comprising at least five or more Transcription activator-like effector (TALE) monomers and at least one or more half-monomers specifically ordered to target the genomic locus of interest, and a C-terminal capping region, wherein the polypeptide includes at least one or more effector domains, and wherein the polypeptide is encoded by and translated from a codon optimized nucleic acid molecule so that the polypeptide preferentially binds to the DNA of the genomic locus. |
申请公布号 |
EP2785831(A4) |
申请公布日期 |
2015.09.09 |
申请号 |
EP20120853802 |
申请日期 |
2012.11.30 |
申请人 |
THE BROAD INSTITUTE, INC.;MASSACHUSETTS INSTITUTE OF TECHNOLOGY;PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
CONG, LE;LOCASCIO, SAM;ZHANG, FENG |
分类号 |
C12Q1/68;C07K14/03;C07K14/195;C07K14/47;C12N5/07;C12N5/071;C12N9/12;C12N9/22;C12N15/63 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|