| 摘要 |
Preparations are disclosed comprising β-glycosyltransferase; UDP-glucosamine and/or a UDP derivative selected from the group consisting of UDP-glucose and UDP-azidoglucose; and an endonuclease having an amino acid sequence at least 95% identical to an enzyme selected from the group consisting of AbaSI, AbaAI, AbaCI, AbaDI, AbaTI, AbaUI, AcaPI, and PxyI. The preparation may also contain an oxidizing agent and/or a reducing agent. Also claimed are methods for differentiating 5-(β-glucosyloxymethyl) cytosine from 5-(β-2-glucosaminyloxymethyl) cytosine in a nucleic acid comprising reacting the nucleic acid with an endonuclease capable of cleaving a nucleic acid at a glucosylated nucleotide but not at a glucosaminylated nucleotide. Also claimed are methods for placing off switches and on switches for endonuclease cleavage of a nucleic acid containing modified nucleotides comprising reacting the nucleic acid alternatively with UDP-glucosamine and UDP-glucose. Related kits are also claimed. |
