IMPROVED ACYLTRANSFERASE POLYNUCLEOTIDES, POLYPEPTIDES, AND METHODS OF USE

摘要

The invention provides chimeric DGAT1 proteins comprising: a) at their N-terminal ends, an N-terminal portion of a first DGAT1 protein, and b) at their C-terminal ends, a C-terminal portion of a second DGAT1 protein. The chimeric DGAT proteins show enhanced activity relative to at least one of the first DGAT1 protein and the second DGAT1 protein. The chimeric DGAT proteins of the invention can be expressed in cells to increase cellular lipid accumulation and/or modify the cellular lipid profile. The invention also provides polynucleotides encoding the chimeric DGAT1 proteins, cells and compositions comprising the polynucleotides or chimeric DGAT1 proteins, and methods using the chimeric DGAT1 proteins to produce oil.