Directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of defined enzyme and metabolite mixtures

摘要

The present invention provides a method for directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of defined enzyme and metabolite mixtures, enzyme and metabolite materials obtained by such method and suitable devices therefore.

主权项

1. A semisterile culture method for producing an enzyme mixture by a mixed culture of microorganisms optimized for the degradation of one or more target substrates, the method comprising
(a) cultivating an inoculum of a mixed culture of microorganisms in a solid-phase bioreactor with one or more solid inducer substrates, wherein at least two microorganisms are selected from A. niger, A. tubingensis and Neurospora intermedia, and wherein at least one solid inducer substrate is rape extraction material, (b) controlling the water activity during the cultivation to force selection pressure on the growing mixed culture of microorganisms; and (c) adding one or more solid target substrates to the mixed culture in the bioreactor at one or more later time points during the course of the cultivation, wherein the solid target substrates are different from the inducer substrates and whereby producing the enzyme mixture optimized for the degradation of one or more target substrates.