Methods of using lung or bronchial epithelial cells to identify bitter taste modulators

摘要

Provided herein are cell lines and assays that can be utilized to identify taste receptor modulators.

主权项

1. A method for identifying a candidate bitter taste modulator for potency testing comprising:
a) contacting a cell with a bitter tastant and a test compound, wherein the cell is a lung or bronchial epithelial cell derived from airway tissue and endogenously expresses a bitter taste receptor; b) measuring bitter taste receptor activity, wherein a change in bitter taste receptor activity by the bitter tastant indicates modulation of the bitter taste receptor by the test compound, thus identifying a bitter taste modulator, and c) identifying a candidate bitter taste modulator for potency testing.