Apparatuses and compositions for cryopreservation of cellular monolayers

摘要

Provided are apparatuses for cryopreserving cells which include a vessel comprising a biocompatible substrate, wherein the vessel further comprises an interior and an exterior, and a mechanical ice nucleating device disposed in or on the vessel interior for initiating ice crystal formation. Also provided are kits comprising one or more apparatuses for cryopreserving cells and a biopreservation medium. Further provided are compositions comprising a vessel for holding cells, a mechanical ice nucleating device, a biopreservation medium, and cells disposed in the vessel. The apparatuses, kits, and compositions of the invention can optionally include an insulating material which is disposed on all or a portion of the vessel.

主权项

1. An apparatus for cryopreserving a plurality of cell monolayers for high throughput screening, the apparatus comprising:
a multiwell tissue culture vessel comprising a biocompatible substrate, wherein each well of the multiwell vessel comprises an interior and an exterior; and, a plurality of mechanical ice nucleating devices arranged so that at least one mechanical ice nucleating device is disposed in the interior of each of a plurality of wells of the multiwell vessel, the mechanical ice nucleating device comprising a three-dimensional protrusion attached to a separable cover or a separable insert for the multiwell vessel such that the protrusion occupies the interior space of the well when the cover or the insert is placed on the multiwell vessel.