Purification of immunoglobulins

摘要

The present invention pertains to improved methods of using dye-ligand affinity chromatography for the isolation of antibodies or proteins comprising an antibody fragment (such as Fc fusion proteins) from a mixture of undesirable contaminants. In particular, the use of an organic polymer such as polyethylene glycol (PEG) in the elution phase of an antibody/dye-ligand chromatography isolation procedure results in improved separation of target antibodies from undesirable contaminants. The methods described herein are particularly useful in separating or removing antibody aggregates, misfolded antibodies, and virus contaminants from target antibodies.

主权项

1. A method of separating an antibody or a protein comprising an antibody fragment from one or more contaminants wherein said method comprises:
a) contacting said antibody or protein and said one or more contaminants with a dye-ligand chromatography media; b) contacting said dye-ligand chromatography media with an organic polymer; and c) after steps a) and b), removing said antibody or said protein from contact with said dye-ligand chromatography media, wherein the molecular weight of the organic polymer is from 100 dalton to about 20,000 dalton; and wherein contacting the dye-ligand chromatography media with the organic polymer according to step b) enhances separation of the antibody or protein from the one or more contaminants as compared to separation of the antibody or protein from the one or more contaminants without contacting the dye-ligand chromatography media with the organic polymer according to step b).