| 摘要 |
A microfluidic apparatus method and associated applications utilize and apply to a formalin fixed paraffin embedded (FFPE) tissue sample and performing a liquid liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid liquid extraction process vessel a nucleic acid purification process component and a nucleic acid amplification reactor. A liquid liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid liquid extraction process and a nucleic acid purification process including a dedicated liquid liquid extraction process vessel an immiscible liquid or a precursor phase thereof disposed in the vessel a nucleic acid purification process component a nucleic acid amplification reactor fluidically and a supply of reagents suitable to enable the liquid liquid extraction process and the nucleic acid purification process. |
