A METHOD FOR FABRICATING BIO-ARTIFICIAL SKIN GRAFT BY LOADING CELLS ON CHOLECYST EXTRACELLULAR MATRIX FOR WOUND HEALING APPLICATIONS

摘要

This invention relates to a process for the preparation of an artificial skin-graft comprising the steps of providing HaCaT cells (immortal keratinocyte cell line), adding trypsin thereto followed by incubation of the cells to detach the cells, followed by neutralisation of the trypsin, isolating scaffolds from cholecyst derived extracellular matrix and subjecting the same to sterilization followed by seeding the HaCaT cells on the scaffolds and maintaining the HaCaT culture on the scaffolds to obtain the skin-graft.