PERFORMANCE ENHANCED AND TEMPERATURE RESISTANT PROTEASE VARIANTS

摘要

Proteases comprising an amino acid sequence that is at least 70% identical to the amino acid sequence given in SEQ ID NO: 1 over its total length and that have R99E or R99D amino acid substitution in the numbering according to SEQ ID NO: 1 in combination with at least two additional amino acid substitutions selected from the group consisting of S3T V4I and V199I show a very good purification performance especially for blood containing fouling and excellent temperature stability.