THERMAL CYCLER AND CONTROL METHOD OF THERMAL CYCLER

摘要

A thermal cycler includes an attachment unit having an insertion opening for insertion of a reaction container including a channel filled with a reaction solution containing reverse transcriptase enzyme and a liquid having a lower specific gravity than that of the reaction solution and being immiscible with the reaction solution, a first heating unit that heats a first region of the channel, a second heating unit that heats a second region of the channel nearer the insertion opening than the first region, a drive mechanism that switches arrangement of the attachment unit, the first heating unit, and the second heating unit between a first arrangement and a second arrangement, and a control unit that controls the first heating unit to be at a temperature at which reverse transcription reaction progresses and the second heating unit to be at a temperature at which the reverse transcriptase enzyme is not deactivated.

主权项

1. A thermal cycler for conducting a polymerase chain reaction comprising:
a holder configured to hold a removable reaction container filled with reaction solution containing target DNA, reverse transcriptase enzyme, primer and a second liquid having a smaller specific gravity than the first liquid and being immiscible with the reaction solution, the removable reaction container including a channel configured to move a reaction solution; a first heating unit configured to heat a first region of the channel; a second heating unit configured to heat a second region of the channel different from the first region; and a driving unit configured to rotate the holder, the first heating unit and the second heating unit about a rotational axis between a first arrangement in which a lowermost position of the channel is located within the first region and a second arrangement in which the lowermost position of the channel is located within the second region.