METAL NANOPARTICLE-APTAMER CONJUGATES FOR DETECTION OF SMALL MOLECULES AND IN-THE-FIELD USE THEREOF

摘要

A method of detecting a presence of an analyte in a sample. The method includes selecting an aptamer selective to the analyte and binding the aptamer to a nanoparticle. The nanoparticles having a free state are perceived as a first color and an aggregate state are perceived as a second color. The sample is introduced to the nanoparticle-bound aptamers, and aggregation of the nanoparticles is promoted. A colorimetric change is analyzed, wherein the aggregate state of the nanoparticles is achieved in the presence of the analyte in the sample.

主权项

1. A method of detecting a presence of an analyte in a sample, the method comprising:
selecting an aptamer selective to the analyte; binding the selected aptamer to a nanoparticle, the nanoparticles having a free state perceived as a first color and an aggregate state perceived as a second color; introducing the sample to the nanoparticle-bound aptamers; promoting the aggregate state of the nanoparticles; and analyzing a colorimetric change, wherein the aggregate state of the nanoparticles is achieved in the presence of the analyte in the sample.