METHODS AND COMPOSITIONS FOR TREATING ALZHEIMER'S DISEASE

摘要

Aspects of the invention relate to methods and compositions for treating Alzheimer's disease (AD). In some embodiments, the invention provides methods for screening and identifying compounds that selectively inhibit the targeting of the insulin-Akt signaling pathway by Aβ oligomers.

主权项

1. A method of identifying a compound that reduces β-amyloid (Aβ) inhibition of Akt activation by PDK1, the method comprising:
contacting a first compound with a preparation comprising an Akt kinase domain, a PDK1 kinase domain, and an Aβ polypeptide; determining a first amount of active Akt; and comparing the first amount to a reference amount of active Akt in the absence of the first compound, wherein a significantly higher amount of active Akt in the presence of the first compound identifies the first compound as a candidate for reducing Aβ mediated inhibition of Akt activation by PDK1.