发明名称 |
EXPRESSION CONSTRUCTS AND METHODS FOR SELECTING HOST CELLS EXPRESSING POLYPEPTIDES |
摘要 |
The invention relates to an expression construct for the soluble expression of polypeptides in host cells and for the expression of polypeptides on the surface of host cells, using alternative splicing. The amount of cell membrane expression of polypeptides such as antibodies, antibody fragments and bispecific antibodies can be correlated directly to the amount of soluble polypeptide expressed. In the case of bispecific antibodies, cell membrane expression of heterodimer and homodimer products can be correlated directly to the soluble expression of these products, thereby aiding selection of a desired producer clone. |
申请公布号 |
CA2940880(A1) |
申请公布日期 |
2015.09.03 |
申请号 |
CA20152940880 |
申请日期 |
2015.03.02 |
申请人 |
GLENMARK PHARMACEUTICALS S.A. |
发明人 |
AEBISCHER-GUMY, CHRISTEL;MORETTI, PIERRE;BERTSCHINGER, MARTIN |
分类号 |
C12N15/09 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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