| 发明名称 |
MDCK-DERIVED CELL LINES ADAPTED TO SERUM-FREE CULTURE AND SUSPENSION CULTURE AND METHOD FOR PREPARING VACCINE VIRUS USING THE CELLS |
| 摘要 |
Disclosed is a Madin-Darby canine kidney (MDCK)-derived cell line. The MDCK-derived cell line is derived from MDCK cells deposited under accession number ATCC CCL-34. The MDCK-derived cell line can be prepared by serum-free culture and suspension culture. Preferably, the MDCK-derived cell line has low or no tumorigenicity. The MDCK-derived cell line is preferably selected from MDCK Sky1023, MDCK Sky10234 and MDCK Sky3851. Further disclosed are a culture method for growing the MDCK-derived cells and a method for producing a vaccine virus using the MDCK-derived cells. |
| 申请公布号 |
US2015247128(A1) |
申请公布日期 |
2015.09.03 |
| 申请号 |
US201514711489 |
申请日期 |
2015.05.13 |
| 申请人 |
SK Chemicals Co., Ltd. |
发明人 |
Park Yong Wook;Lee Kun Se;Lee Bong-Yong;Park Mahnhoon;Kim Hun;Kim Yun-Hee;Lee Su-Jeen |
| 分类号 |
C12N7/00;C12N5/071 |
主分类号 |
C12N7/00 |
| 代理机构 |
|
代理人 |
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| 主权项 |
1. A Madin-Darby canine kidney (MDCK)-derived cell line that is derived from MDCK cells deposited under accession number ATCC CCL-34, does not require serum for cell growth and is prepared by suspension culture without the need to be attached to carriers. |
| 地址 |
Suwon KR |
