发明名称 |
FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS |
摘要 |
Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway. |
申请公布号 |
US2015247169(A1) |
申请公布日期 |
2015.09.03 |
申请号 |
US201514715992 |
申请日期 |
2015.05.19 |
申请人 |
Butamax Advanced Biofuels LLC |
发明人 |
DONALDSON Gail K.;Eliot Andrew C.;Flint Dennis;Maggio-Hall Lori Ann;Nagarajan Vasantha |
分类号 |
C12P7/16;C12N9/88;C12N9/10 |
主分类号 |
C12P7/16 |
代理机构 |
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代理人 |
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主权项 |
1. A method of converting a renewable feedstock to a feedstock chemical comprising:
a) providing a recombinant yeast host cell that expresses an engineered biosynthetic isobutanol pathway, wherein the engineered biosynthetic isobutanol pathway comprises an acetolactate synthase (ALS) enzyme, a ketol-acid reductoisomerase (KARI) enzyme, a dihydroxy-acid dehydratase (DHAD) enzyme, a branched chain keto acid decarboxylase enzyme (DC), and an alcohol dehydrogenase (ADH) enzyme, each of which is encoded by a heterologous gene that lacks a mitochondrial targeting sequence; and b) growing the yeast host cells in fermentation medium comprising a renewable feedstock, whereby isobutanol is bioproduced from the renewable feedstock and is capable of being used as a chemical feedstock; wherein the yeast host cells produce 2- to 6-fold more isobutanol when grown on sucrose under aerobic conditions compared to a recombinant yeast host cell lacking the engineered biosynthetic isobutanol pathway. |
地址 |
Wilmington DE US |