Optics Collection and Detection System and Method

摘要

Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.

主权项

1. A chip for monitoring analytical reactions comprising:
an aperture layer comprising an array of nanoscale apertures, each nanoscale aperture forming a nanoscale well for receiving a fluid including at least two different fluorescent species; a fluidic layer above the aperture layer and in fluidic contact with the nanoscale wells; a waveguide layer below the aperture layer and providing illumination light to the nanoscale wells, the waveguide layer comprising an array of waveguides, each waveguide extending across a plurality of nanoscale wells; a transmission layer below the waveguide layer; and a detector layer below the transmission layer and comprising a detector, wherein: the transmission layer transmits light emitted from the fluorescent species in the nanoscale wells to the detector layer, and the detector receives and detects the emitted light from the nanoscale wells that is transmitted through the transmission layer, wherein for each nanoscale well, the detector has one or two pixels that receive emitted light from such nanoscale well, and wherein each pixel resolves spectra of at least two fluorescent species.