METHODS FOR MULTIPLEX ANALYTICAL MEASUREMENTS IN SINGLE CELLS OF SOLID TISSUES

摘要

The invention provides a method for the isolation of a single cell embedded in a tissue while preserving the state of molecules of the cell, and therefore allows for transformation of a single target cell in live tissue into a format that can be evaluated using analytical methods.

主权项

1. A method of lysing a single cell present in a tissue, the method comprising:
a) identifying a cell from a tissue; b) contacting a detergent-containing lysis buffer with the intracellular space of the identified cell; c) allowing the lysis buffer to spread within the intracellular space of the identified cell for a period of time, wherein the cell is lysed from the inside of the cell; and d) collecting the lysate.