METHODS FOR ENHANCING HEMATOPOIETIC STEM/PROGENITOR CELL ENGRAFTMENT

摘要

Described herein are methods for enhancing engraftment of hematopoietic stem and progenitor cells using compounds identified using a zebrafish model of hematopoietic cell engraftment. The compounds can be used to treat hematopoietic stem cells ex vivo prior to transplantation of the cells. Alternatively, the compounds can be administered to an individual undergoing cell transplantation.

主权项

1. A method for enhancing hematopoietic cell engraftment in a subject, the method comprising:
(a) contacting a population of hematopoietic cells with a compound selected from the group consisting of: 1,2-didecanoyl-glycerol (10:0), 11,12 Epoxyeicosatrienoic acid (11,12 EET), 1-hexadecyl-2arachidonoyl glycerol, 6-formylindolo[3,2-B]carbazole, 9-cis-retinoic acid, anandamide, carbacyclin, clofibrate, carbamyl PAF (C-PAF), Prostaglandin B2,5-hydroxydecanoate, and 1,2-dioctanoyl-sn-glycerol (DOG); and (b) administering treated cells from step (a) to a subject in need thereof,wherein the engraftment of the hematopoietic cells treated with the compound is increased compared to engraftment of a population of untreated hematopoietic cells.