| 摘要 |
It was discovered that, in a plant cell, a marker gene inserted into the genomic DNA by homologous recombination and having nuclease recognition sites added to both ends thereof can be removed by the corresponding nuclease, and the recognition sites can be removed without leaving a trace by matching the sequence of at least 30 nucleotides adjacent to the nuclease recognition sites. Thus, it is possible, in a method for introducing a mutation into target DNA on a plant cell genome by homologous recombination, to select, in a stable manner and as an indicator of expression of the marker gene, plant cells into which this mutation has been introduced, and it is possible to remove unnecessary sequences such as marker genes and to introduce only the necessary mutation into the target DNA in the selected cells. |
