| 摘要 |
The invention provides a method of detecting a non-light emitting target particle in a solution using a scanning molecule counting method without applying a light emitting label. According to the invention, there is provided a target particle detection method of detecting a non-light emitting particle using an optical system of a confocal microscope or a multi-photon microscope, including the steps of: binding a non-light emitting observation particle and a solid phase carrier to each other through a target particle which is a detection target in a solution or at an interface of the solution; (b) removing observation particles in a free state after the step (a); (c) separating the observation particles from the complexes after the step (b); (d) preparing a sample solution for measurement in which the separated observation particles are dispersed after the step (c); and (e) detecting the observation particles in the sample solution for measurement prepared in the step (d) using an inverted scanning molecule counting method. |
