Stepwise assembled capture compounds comprising a cleavable function and method for isolating and/or characterizing biomolecules or biomolecule fragments, in particular proteins or protein fragments, of complex mixtures

摘要

The invention relates to a process for isolating and/or characterising biomolecules and/or biomolecule fragments, in particular proteins and/or protein fragments, comprising the steps:
provision of an immobilized compound IC through the interaction of a capture compound CC and the target compound T and a carrier compound CCB, carrying out a division step, in which a cleavable function F is cleaved, and a division compound DC is produced, and
isolation and/or characterisation of said division compound DC. The invention relates further to a carrier compound CCB, a capture compound CC, a precursor compound PC and a division compound DC.