| 摘要 |
<p>The present invention relates to a method of clonally sorting and isolating living cells, which have a specific ribonucleic acid (RNA) molecule in common, wherein the ribonucleic acid molecule is the transcriptional product of at least two different genomic regions, the method comprising the steps of:
a. providing a sample comprising said living cells;
b. providing at least one labeled probe set, each probe set comprising
i. a first probe which is specific for a region of the ribonucleic acid stemming from a first genomic region and,
ii. a second probe which is specific for a region of the ribonucleic acid stemming from a second genomic region and,
c. transfecting said cells with said probe set such that hybridization to said regions of the ribonucleic acid molecule may occur and if said hybridization occurs the probes lie adjacent to one another on said ribonucleic acid molecule;
d. detecting hybridization of said probe set to said nucleic acid regions; and
e. sorting the cells depending on how hybridization to said regions has occurred.</p> |
