Process for preparing FGF21 with low degree of O-glycosylation

摘要

Expression of FGF21 in a S. cerevisiae pmt2 knock out strain reduces the O-glycosylation significantly and does not decrease the expression level. Expression in mkc7 knock out strain can decrease degradation of FGF21 and analogues thereof. Point mutations at position R17 and R36 can decrease degradation of FGF21 and analogues thereof when they are expressed in yeast.

主权项

1. A process for preparing FGF21 having a sequence of SEQ ID NO:1 and analogues thereof, comprising the steps of:
a. introducing an expression vector comprising a DNA molecule encoding FGF21 or analogues thereof into a Saccharomyces cerevisiae yeast strain having a ymt2 knockout; b. culturing said yeast under conditions for expressing and secreting FGF21 or analogues thereof.