Systems using cell culture for production of isoprene

摘要

The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. Additionally, the invention provides methods of producing isoprene by culturing cells under conditions suitable for isoprene production while maintaining cell viability and/or metabolic activity.

主权项

1. A method of producing isoprene, the method comprising:
a) culturing cells comprising one or more nucleic acids encoding an isoprene synthase polypeptide for production of isoprene; b) producing isoprene, wherein the cells produce greater than 400 nmole/gwcm/hour of isoprene, and wherein the carbon dioxide evolution rate of the cells is greater than 1×10−18 mmol/L/hour; and c) recovering the isoprene made in step (b).