发明名称 |
Polymerase chain reaction product-cloning vector suitable to its easy production and method for producing the same |
摘要 |
The present invention relates to a PCR product cloning vector applicable directly to the cloning of a PCR product. More precisely, the present invention relates to a PCR product cloning vector designed to be effective in use for blue/white colony selection and produced based on the restriction enzyme treatment process to generate non-complementary unpaired single overhang and a method for producing the same. According to the method of the present invention, the PCR product cloning vector designed to be advantageous for blue/white cloning selection can be produced with improved efficiency compared with the convention method. |
申请公布号 |
US9121023(B2) |
申请公布日期 |
2015.09.01 |
申请号 |
US200812740003 |
申请日期 |
2008.10.29 |
申请人 |
INTRON BIOTECHNOLOGY, INC. |
发明人 |
Yoon Seongjun;Jun SooYoun;Kang SangHyeon |
分类号 |
C12N15/00;C12N15/64;C12N15/70 |
主分类号 |
C12N15/00 |
代理机构 |
Ballard Spahr LLP |
代理人 |
Ballard Spahr LLP |
主权项 |
1. A method of making a linearized double-stranded DNA vector fragment for direct cloning of a polymerase chain reaction (PCR) product by Xcm I restriction enzyme treatment of a circular parental alpha-peptide gene sequence containing vector comprising the nucleotide sequence of SEQ ID NO:4 comprising two Xcm I restriction enzyme recognition sequences, wherein Xcm I restriction enzyme treatment generates a double-stranded DNA vector fragment having non-complementary unpaired single overhang ends in the recognition site in different strands of the double-stranded DNA structure. |
地址 |
Sungnam-Si KR |