In situ generation of ozone for mass spectrometers

摘要

In some embodiments, a mass spectrometer capable of performing OzID is disclosed that can provide ozone in situ within an evacuated chamber of the spectrometer, e.g., within a collision cell or within the vacuum chamber of the mass spectrometer. In some embodiments, a corona discharge generated within the evacuated chamber can be employed to convert an ozone precursor delivered to the chamber into ozone.

主权项

1. A mass spectrometer system comprising:
(i) means for ionizing the compound to provide ions; (ii) means for selecting ions of a given mass-to-charge ratio; (iii) means for allowing the selected ions to react with ozone to give ozone-induced dissociation fragment ions; (iv) means for mass analyzing and detecting the ozone induced fragment ions formed in step (iii); and (v) means for determining the number of and position(s) of any carbon-carbon double bonds in the compound based on the difference between the mass-to-charge ratio of the ions selected by the ion selection means, and the mass-to-charge ratio of one or more of the ozone-induced dissociation fragment ions formed from the selected ions formed by the reaction means; wherein the ozone is generated within the vacuum chamber of the mass spectrometer system, at or near the ion/molecule reaction region where reactions between ions and ozone can take place.