Dynamic thermoresponsive nanoparticles for stabilization of enzymes at high temperatures

摘要

The present invention provides a thermoresponsive nanoparticle useful for the stabilization of enzymes in environments having a temperature greater than thirty degrees Centigrade. The thermoresponsive nanoparticle has (a) a functionalized enzyme conjugate having one or more enzymes or biological catalysts, the enzymes or biological catalysts are modified with palmitic acid N-hydroxysuccinimide ester and acryclic acid N-hydroxysuccinimide ester, and (b) a thermally responsive polymer, wherein the functionalized enzyme conjugate is encapsulated within the thermally responsive polymer. A nanocatalyst is provided that has one or more proteins. The proteins are covalently immobilized and encapsulated within a thermally responsive polymer shell. The proteins are one or more enzymes or biological catalysts. A method for protecting the proteins is also set forth.

主权项

1. A method for protecting nanocatalysts comprising one or more proteins from environmental conditions, said method comprising:
immobilizing one or more nanocatalysts comprising the proteins and completely encapsulating said one or more immobilized nanocatalysts comprising the proteins within one or more thermally responsive polymeric nanoparticles by raising the temperature above the lower critical solution temperature of said thermally responsive polymeric nanoparticles during an initiation process wherein said nanoparticles undergo a conformational change when heated whereby protecting said nanocatalysts comprising the proteins from temperatures greater than 30 degrees Centigrade.