Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell

摘要

The present invention relates to: a method for producing an intermediate mesoderm cell from a human pluripotent stem cell, comprising a step of culturing the human pluripotent stem cell in a medium containing Activin A and Wnt or a functional equivalent of Wnt and a step of culturing cells in a medium containing BMP and Wnt or a functional equivalent of Wnt; to a method for producing a metanephric cell from the intermediate mesoderm cell produced by the first method; to a human pluripotent stem cell having a foreign reporter gene in the chromosome wherein the gene is expressed interlocked with the expression of endogenous OSR1; to a method for screening for an inducer for differentiation into intermediate mesoderm using the human pluripotent stem cell; and to a kit for inducing the differentiation into an intermediate mesoderm cell.

主权项

1. A method for producing an intermediate mesoderm cell from a human pluripotent stem cell, comprising the following steps (i) and (ii) of:
(i) culturing the human pluripotent stem cell in a medium containing Activin A and Wnt or a functional equivalent of Wnt, and then (ii) culturing the cell obtained in the step (i) in a medium containing BMP and Wnt or a functional equivalent of Wnt, and (iii) obtaining intermediate mesoderm cells, wherein the culture in the step (i) is performed for a culture period ranging from 2 to 5 days, and wherein the intermediate mesoderm cell is an OSR1-positive cell and is identified by detection of one or more markers selected from the group consisting of: PAX2, WT1, EYA1, and SIX2.