Chinese herbal aqueous extract having anti-anxiety activities and method of in vitro evaluating the same

摘要

A Chinese herbal aqueous extract having anti-anxiety activities and a method of in vitro evaluating the same are disclosed. Neural cells and/or glial cells are cultured in a medium containing the Chinese herbal aqueous extract, and then subjected to an electric pulse treatment to form epileptic cells. The epileptic cells are applied on evaluation of at least one criterion of anti-anxiety activities of the Chinese herbal aqueous extract.

主权项

1. A method of in vitro evaluating Chinese herbal aqueous extract with anti-anxiety activity, comprising:
heating a raw material of a Chinese herbal medicine sample with water and to keep them boiling for 105 minutes, so as to obtain a Chinese herbal aqueous extract, wherein the Chinese herbal aqueous extract is a test sample; culturing a neural cell and/or a glial cell in a testing medium that includes the test sample or not for 12 hours to 36 hours, wherein the neural cell and/or the glial cell includes a neural cell line and/or a glial cell line; applying an electric pulse treatment on the neural cell and/or the glial cell, allowing the neural cell and/or the glial cell to be suffered with a hyper-excitatory electrical potential injury, thereby forming an epileptic cell; detecting at least one anti-anxiety parameter of the epileptic cell, the anti-anxiety parameter comprises a cell viability, a percentage of reactive oxygen species (ROS)-generated cells and a percentage of apoptotic cells, wherein at least one test value is defined to the at least one anti-anxiety parameter obtained from the epileptic cell cultured with the test sample, and at least one reference value is defined to the at least one anti-anxiety parameter obtained from the epileptic cell cultivated without the test sample; and comparing the test value with the reference value, so as to determine that the test sample has the anti-anxiety activity based on when the cell viability is more than the reference value, as well as the percentage of the ROS-generated cells and the percentage of apoptotic cells are less than the reference value.