PLANT CELL IN WHICH MUTATION HAS BEEN INTRODUCED TO TARGET DNA, AND METHOD FOR PRODUCING SAME

摘要

This method for producing a plant cell to which a mutation has been introduced to target DNA includes: a step for introducing into a plant cell a DNA construct that contains DNA homologous to the target DNA and in which a PiggyBac transposon containing a marker gene has been introduced to the homologous DNA and a desired mutation has been introduced; a step for selecting, with the expression of the marker gene as an indicator, plant cells to which the target DNA has been introduced by means of homologous recombination of the mutation and the PiggyBac transposon; and a step for eliminating the PiggyBac transposon from the target DNA by means of constitutive expression of the PiggyBac transposon in the cells selected in the aforementioned step.