PIPETTE AND METHOD FOR PIPETTING

摘要

A pipette has a first chamber and a second chamber that connects to the first chamber and extends with an outlet channel to a pipette tip. The chambers are separated from one another by a longitudinally displaceable piston having a piston non-return valve. The second chamber has a chamber non-return valve which is arranged upstream of the outlet channel. An associated method includes recirculating the liquid in the first chamber, filling the first chamber with the liquid via a direct feed line, filling the second chamber with the liquid from the first chamber by pulling back the piston in the filling position toward the first chamber with the piston non-return valve open and with the chamber non-return valve closed, and emptying the second chamber by advancing the piston in the emptying position towards the outlet channel, with the piston non-return valve closed and the chamber non-return valve open.

主权项

1. A pipette comprising:
a first chamber, arranged in a housing, and a second chamber, also arranged in the housing, which connects to the first chamber and extends through an outlet channel to a pipette tip, wherein the first and second chambers are separated from one another by a longitudinally displaceable piston having a piston non-return valve, wherein the second chamber comprises a chamber non-return valve, which is arranged upstream of the outlet channel, wherein the first chamber is configured to be filled with liquid via a direct feed line, and wherein the first chamber is connected via the feed line and an outlet line to a recirculation circuit of a storage tank for the liquid.