IDENTIFICATION OF ENZYME ACTIVITY THROUGH DETERMINATION OF ITS LOCALIZATION

摘要

A method of determining enzyme activity and identifying and classifying cellular targets, enzymatic pathways, and enzymatic agents involved in regulating metabolism in order to treat pathophysiological disorders. Monitoring enzyme activity is performed via a label-free bio cellular assay or fluorescence imaging. The identified and classified agents are used, together with a therapeutic agent, in the treatment of various metabolism-related diseases.

主权项

1. A method of identifying cellular targets involved in regulating enzyme condensation dynamics, the method comprising the steps of:
providing a cell that has a cellular target; contacting the cell with a molecule that interacts with the cellular target to form a molecule-contacted cell; sequentially exposing the molecule-contacted cell to an enzyme condensation promoting agent and an enzyme condensation disrupting agent; monitoring the state of enzyme condensation of the molecule-contacted cell after exposure to the enzyme condensation promoting agent and the enzyme condensation disrupting agent; and determining the molecule's ability to modulate the state of enzyme condensation thereby identifying cellular targets involved in regulating enzyme condensation dynamics.