Method for Compensating for the Aging of a Reagent During Fluorescence Measurements Carried out on Particles, and Biological Analysis Device Implementing the Method

摘要

The present invention relates to a method for compensating for the breakdown of a reagent stored in an aqueous phase comprising at least one fluorescent compound and enabling the identification of particles, including the steps of: (i) measuring the fluorescence level FLUOm(t) of particles marked with said reagent; (ii) measuring the absorbance at at least one wavelength of a solution of said reagent, at a time t close to the time of said fluorescence level FLUOm(t) measurements, so as to determine at least one current optical density DO(t) of the reagent; and (iii) calculating a correction of the fluorescent level measurements using said at least one current optical density DO(t) and at least one initial optical density DO(0) of the reagent that has not been broken down. The invention also relates to a biological analysis device implementing the method.

主权项

1. A method for compensating for the degradation of a reagent stored in an aqueous phase comprising at least one fluorescent compound and allowing identification of particles, comprising a step of measurement (62) of the fluorescence level FLUOm(t) on particles labeled with said reagent, characterized in that it further comprises steps of:
carrying out absorbance measurements (61) at at least one wavelength on a solution of said reagent, at a time point t close to that of said measurements of the fluorescence level FLUOm(t), so as to determine at least one current optical density OD(t) of the reagent, and, calculating a correction (63) of the measurements of the fluorescence level using said current optical density or densities OD(t) and at least one initial optical density OD(0) of the degradation-free reagent.