METHOD FOR OBTAINING AND DETECTING A MARKER OF OBJECTS TO BE IDENTIFIED, RELATED MARKER, AUTHENTICATION METHOD AND VERIFICATION METHOD

摘要

Procedure for obtaining a marker of objects to be identified, comprising at least one polymorphic DNA fragment and preferably a plurality of polymorphic DNA fragments, such as microsatellite or single base polymorphism, modified in their three-dimensional structure, adsorbed to metallic nanoparticles and micro encapsulated, which are active to Raman radiation. Said marker is also protected; as well as the marker detection method; the method to instantly authenticate marked objects with the said marker; the method to verify the marked objects with the said marker and the method for incorporating the said marker to the desired object to be identified.

主权项

1. Procedure for obtaining a marker of objects to be identified, comprising at least a first step of selecting a living being for DNA extraction, and an eighth step of determining and correcting the degree of fluidity of the solution; comprising also;
a second step, of including the obtained sample in a solution containing between 9 and 10.2 mM Tris-HCl; between 0.95 and 0.11 EDTA; 20% SDS (w/v) and from 9.8 to 10.3 mg/ml Proteinase K, proceeding to the purification with phenol/chloroform at a ratio of 10:9 (v/v); a third step, of amplifying short tandem polymorphic fragments (STRs) or single nucleotide polymorphisms (SNPs) present in the DNA sample; fourth step, of determining the allelic variants of polymorphic fragments chosen; a fifth step, of modifying the three dimensional structure of polymorphic STRs/SNPs fragments and adsorption to nanoparticles of at least one metal; a sixth step, of concentration and microencapsulation of polymorphic fragments; and a seventh step, of solubilization of the DNA microspheres or microencapsulated DNA to be detected by Raman spectroscopy.