| 摘要 |
A method of fractionating semen into X- and Y-chromosome-bearing spermatozoa comprising: layering an aliquot of semen on a disaccharide medium prepared as a density gradient having a concentration range of less than 15 % w/v, wherein the pH range of the solution is maintained in the range of from 5.5 to 9.0; and centrifuging the medium to obtain at least two distinct fractions of semen, a first contained in a lower, denser layer and a second contained in at least one upper, lighter layer, wherein the X-chromosome-bearing spermatozoa are enriched in the first fraction and the Y-chromosome-bearing spermatozoa are enriched in the second fraction; and separating the upper layer from the lower layer. |
