Deducing Exon Connectivity by RNA-Templated DNA Ligation/Sequencing

摘要

A technology is described that is capable of generating high-throughput sequencing (HTS) read length DNA products to accurately and reliably provide exon connectivity information for alternatively spliced isoforms. The method is not limited by the initial size of the isoform as the technology removes the template oligonucleotide sequence and a newly formed full length ligated product provides an HTS-compatible read length sequence that comprises information that corresponds to the consecutive order of the exons in the original template oligonucleotide.

主权项

1. A composition comprising a ligamer comprising a plurality of complementarity regions, wherein at least two of said plurality of complementary regions are separated by a central region, wherein said central region has an exon-specific barcode sequence comprising an identifier code for a specific exon, wherein said at least two of said plurality of complementarity regions are complementary to the flanking regions of said specific exon.