发明名称 |
CD20- AND EGFR-BINDING PROTEINS WITH ENHANCED STABILITY |
摘要 |
The invention provides CD20-binding proteins and EGFR-binding proteins having a reduced tendency to aggregate. Compositions and methods of use are also provided. |
申请公布号 |
US2015239977(A1) |
申请公布日期 |
2015.08.27 |
申请号 |
US201314431830 |
申请日期 |
2013.09.27 |
申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
发明人 |
Trout Bernhardt Levy;Schneider Curtiss Paul;Agrawal Neeraj Jagdish |
分类号 |
C07K16/28;A61J1/06 |
主分类号 |
C07K16/28 |
代理机构 |
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代理人 |
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主权项 |
1. A CD20-binding protein comprising:
a light chain domain having an amino acid sequence of SEQ ID NO:1 modified to include at least one amino acid substitution selected from the group consisting of: a neutral polar amino acid at V3, a neutral polar amino acid at 9, a neutral polar amino acid at I10, a neutral polar amino acid at V59 and a negatively charged polar amino acid at L153; and/or a heavy chain domain having an amino acid sequence of SEQ ID NO:2 modified to include an amino acid substitution selected from the group consisting of a neutral polar amino acid at Y101 and a neutral polar amino acid at L178; and/or an Fc domain having an amino acid sequence of SEQ ID NO:3 modified to include at least one amino acid substitution selected from the group consisting of: a positive polar amino acid at position L15, a positive polar amino acid at position I33, a positive polar amino acid at position V62, and a positive polar amino acid at position L89. |
地址 |
Cambridge MA US |