发明名称 CD20- AND EGFR-BINDING PROTEINS WITH ENHANCED STABILITY
摘要 The invention provides CD20-binding proteins and EGFR-binding proteins having a reduced tendency to aggregate. Compositions and methods of use are also provided.
申请公布号 US2015239977(A1) 申请公布日期 2015.08.27
申请号 US201314431830 申请日期 2013.09.27
申请人 MASSACHUSETTS INSTITUTE OF TECHNOLOGY 发明人 Trout Bernhardt Levy;Schneider Curtiss Paul;Agrawal Neeraj Jagdish
分类号 C07K16/28;A61J1/06 主分类号 C07K16/28
代理机构 代理人
主权项 1. A CD20-binding protein comprising: a light chain domain having an amino acid sequence of SEQ ID NO:1 modified to include at least one amino acid substitution selected from the group consisting of: a neutral polar amino acid at V3, a neutral polar amino acid at 9, a neutral polar amino acid at I10, a neutral polar amino acid at V59 and a negatively charged polar amino acid at L153; and/or a heavy chain domain having an amino acid sequence of SEQ ID NO:2 modified to include an amino acid substitution selected from the group consisting of a neutral polar amino acid at Y101 and a neutral polar amino acid at L178; and/or an Fc domain having an amino acid sequence of SEQ ID NO:3 modified to include at least one amino acid substitution selected from the group consisting of: a positive polar amino acid at position L15, a positive polar amino acid at position I33, a positive polar amino acid at position V62, and a positive polar amino acid at position L89.
地址 Cambridge MA US