METHOD FOR THE PRODUCTION OF 3-HYDROXY-3-METHYLBUTYRIC ACID FROM ACETONE AND AN ACTIVATED ACETYL COMPOUND

摘要

Described is a method for the production of 3-hydroxy-3-methylbutyric acid by enzyme-catalyzed covalent bond formation between the carbon atom of the oxo group of acetone and the methyl group of a compound which provides an activated acetyl group. Also described are recombinant organisms which produce 3-hydroxy-3-methylbutyric acid, and related compositions and methods.

主权项

1. A method for the production of 3-hydroxy-3-methylbutyric acid comprising an enzyme-catalyzed reaction between (a) acetone and (b) a compound of formula (I) which provides an activated acetyl group: wherein X is S—CH2—CH2—NH—CO—CH2—CH2—NH—CO—CH(OH)—C(CH3)2—CH2—O—PO2H—C10H13N5O7P (coenzyme A), wherein the enzyme-catalyzed reaction is performed by an enzyme having the activity of a HMG CoA synthase (EC 2.3.3.10) and which catalyzes the formation of a covalent bond between the carbon atom of the oxo (C═O) group of the acetone and the carbon atom (C2) corresponding to the methyl group of said compound of formula (I).