发明名称 |
Detection of an Amplification Reaction Product Using pH-sensitive Dyes |
摘要 |
Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer. |
申请公布号 |
US2015240293(A1) |
申请公布日期 |
2015.08.27 |
申请号 |
US201514680721 |
申请日期 |
2015.04.07 |
申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
Tanner Nathan;Zhang Yinhua;Evans Thomas C. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
Ipswich MA US |