PRODUCTION OF RED BLOOD CELLS AND PLATELETS FROM STEM CELLS
摘要
This disclosure provides methods of making a megakaryocyte-erythroid progenitor cell (MEP), comprising differentiating a stem cell into a MEP in culture in the presence of an aryl hydrocarbon receptor (AhR) agonist. In some embodiments the stem cell is a pluripotent stem cell. In some embodiments the MEP co-expresses CD41 and CD235. In some embodiments the number of MEPs produced in the culture increases exponentially. Methods of making a red blood cell (RBC) by culturing a MEP in the presence of an AhR agonist are also provided. Methods of making a megakaryocyte and/or a platelet, comprising culturing a MEP in the presence of an AhR modulator are also provided. In some embodiments the AhR modulator is an AhR antagonist. This disclosure also provides compositions comprising at least 1 million MEPs per ml and compositions in which at least 50% of the cells are MEPs.
申请公布号
EP2909313(A2)
申请公布日期
2015.08.26
申请号
EP20130829847
申请日期
2013.08.15
申请人
BOSTON MEDICAL CENTER CORPORATION;TRUSTEES OF BOSTON UNIVERSITY
发明人
MURPHY, GEORGE, J.;SHERR, DAVID, H.;ROZELLE, SARAH, S.;SMITH, BRENDEN, W.