发明名称 |
METHODS FOR COUPLING TARGETING PEPTIDES ONTO RECOMBINANT LYSOSOMAL ENZYMES FOR IMPROVED TREATMENTS OF LYSOSOMAL STORAGE DISEASES |
摘要 |
Described herein are methods of making targeting peptides conjugated to recombinant lysosomal enzymes by modifying the amino (N)-terminus and one or more lysine residues on recombinant human lysosomal enzymes using a first crosslinking agent to give rise to first crosslinking agent modified recombinant human lysosomal enzymes, modifying the first amino acid within a short linker at the amino (N)-terminus on a variant IGF-2 peptide using a second crosslinking agent to give rise to a second crosslinking agent modified variant IGF-2 peptide, and then conjugating the first crosslinking agent modified recombinant human lysosomal enzyme to the second crosslinking agent modified variant IGF-2 peptide containing a short linker. Also described herein are conjugates synthesized characterized as having higher affinities for the IGF2/CI-MPR receptor and cellular uptake using the methods disclosed herein. Also described herein are treatment methods using the disclosed conjugates. |
申请公布号 |
EP2714752(A4) |
申请公布日期 |
2015.08.26 |
申请号 |
EP20120793015 |
申请日期 |
2012.05.25 |
申请人 |
AMICUS THERAPEUTICS, INC. |
发明人 |
DO, HUNG |
分类号 |
C08F4/52;A61K38/47;A61K47/48;C07K14/65;C12N9/16;C12N9/24;C12N9/96 |
主分类号 |
C08F4/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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