A THERMOSTABLE SUCROSE AND SUCROSE-6'-PHOSPHATE PHOSPHORYLASE

摘要

The present invention relates to the identification, recombinant expression and characterization of a sucrose and sucrose-6'-phosphate phosphorylase of the thermophilic bacterium Thermoanaerobacterium thermosaccharolyticum. The enzyme has an optimal temperature of about 55°C and has a high specificity for its substrates. More importantly, the sucrose and sucrose-6'-phosphate phosphorylase of the present invention has a half-life of at least 60 hours at the industrially relevant temperature of about 60°C. This enzyme is thus -for example-useful for the industrial production of D-fructose,D-fructose-6-phosphate,alpha-D-glucose -1-phosphate or alpha-D-glucosides.