Methods and compositions for increasing toxin production

摘要

The invention provides methods and compositions (such as for example, culture media) for culturing Clostridium difficile and producing the C. difficile Toxins A and B.

主权项

1. A method for obtaining one or more Clostridium difficile (C. difficilel) toxins comprising the steps of:
(a) preparing an aqueous growth medium comprising soy peptone and D-sorbitol; (b) inoculating the medium with a C. difficile bacterium; (c) culturing the inoculated medium under conditions which facilitate growth of bacterium and toxin production; and (d) isolating the one or more C. difficile toxins from the growth medium.