发明名称 |
Fractionation of oil-bearing microbial biomass |
摘要 |
The invention generally relates to the production of hydrocarbon compositions, such as a lipid, in microorganisms. In particular, the invention provides methods for extracting, recovering, isolating and obtaining a lipid from a microorganism and compositions comprising the lipid. The invention also discloses methods for producing hydrocarbon compositions for use as biodiesel, renewable diesel, jet fuel, and other materials. |
申请公布号 |
US9115332(B2) |
申请公布日期 |
2015.08.25 |
申请号 |
US201314055763 |
申请日期 |
2013.10.16 |
申请人 |
Solazyme, Inc. |
发明人 |
Dillon Harrison F.;Elefant Dan;Day Anthony G.;Franklin Scott;Wittenberg Jon |
分类号 |
C11B1/10;C10L1/02;C12P7/64;C11C3/00 |
主分类号 |
C11B1/10 |
代理机构 |
Alston & Bird LLP |
代理人 |
Alston & Bird LLP |
主权项 |
1. A method of extracting a lipid from recombinant Prototheca cells, the method comprising the steps of:
(a) lysing recombinant Prototheca cells to produce a lysate, wherein the recombinant Prototheca cells:
(i) have not been subjected to a drying step between culturing and lysing; and(ii) comprise at least 10% lipid by dry weight, and wherein the fatty acid profile of the lipid is at least 10% C8-C14 ; (b) treating the lysate with an organic solvent for a period of time, wherein the organic solvent is an alcohol selected from ethanol, isopropanol or butanol; (c) separating the treated lysate into layers comprising a lipid layer and an aqueous layer and, optionally, a lipid:aqueous emulsion layer, and/or a cell pellet; and (d) removing the lipid from the other layer(s). |
地址 |
South San Francisco CA US |