Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue

摘要

A composition and method for generating reagents and the composition of these reagents for the stabilization and preservation of viability of cancer tissue which has been surgically excised and the suspension and/or termination of apoptosis (cell death) by significant modulation of cell metabolism by low molar concentrations of synergistic chemistries and hormonal growth enhancers while maintaining normal gene expression patterns of the surgically excised tissue.

主权项

1. A method of producing a cell viability reagent for a tissue sample, the method comprising:
providing at least one chaotrope; providing a first and a second kosmotrope, wherein the first kosmotrope is glycerol and the second kosmotrope is α,α-trehalose; providing a chelator; providing a buffer; providing an apoptosis reducing substrate, wherein the apoptosis reducing substrate is Leptin; providing a metabolic modulator; mixing the at least one chaotrope, the first and second kosmotrope, the chelator, the buffer, the apoptosis reducing substrate, and the metabolic modulator to produce the cell viability reagent wherein the cell viability reagent is capable of preserving the tissue sample for gene expression analysis, wherein the tissue sample is a surgically excised cancer tissue sample, and wherein a final concentration of the chaotrope is from about 0.1 Molar to about 2 Molar, a final concentration of the first and the second kosmotrope is from about 0.1 Molar to about 2 Molar, a final concentration of the chelator is from about 0.1 Molar to about 2 Molar, and a final concentration of the apoptosis reducing substrate is from about 0.001 Molar to about 0.5 Molar.