Method for detecting gene modifications by means of asymmetrical PCR and blocking agents

摘要

A method of detecting at least one gene modification such as a mutation in a gene includes carrying out an asymmetric polymerase chain reaction (PCR) with a combined use of at least one detectable mutation-specific hybridization probe (sensor probe) and at least one wild-type specific blocking agent which inhibits a binding of the at least one detectable mutation-specific hybridization probe (sensor probe) to a wild-type gene so as to provide at least one of a selective intensification and an amplification of a detection of a gene segment of a mutation gene having a gene modification.

主权项

1. A method of detecting at least one gene modification such as a mutation in a gene, the method comprising:
carrying out an asymmetric polymerase chain reaction (PCR) using a PCR reaction mixture comprising at least one detectable mutation-specific hybridization probe (sensor probe) and at least one wild-type specific blocking agent which inhibits a binding of the at least one detectable mutation-specific hybridization probe (sensor probe) to a wild-type gene so as to provide at least one of a selective intensification and an amplification of a detection of a gene segment of a mutation gene having a gene modification, wherein, the at least one wild-type blocking agent is selected so that the at least one wild-type blocking agent has a higher specificity for an area of a DNA single strand of the wild-type gene, which corresponds to the gene section of the mutation gene having the mutation, compared to the mutation gene.