<i>Amycolatopsis </i>sp. strain and methods of using the same for vanillin production

摘要

This invention provides an Amycolatopsis sp. strain (zhp06), and a method of using the whole cell preparation of the strain for vanillin production. The strain was deposited in China Center for Type Culture Collection on Jul. 26, 2011 with the number of CCTCC NO: M 2011265. Under high concentrations of ferulic acid substrate, the vanillin production by this method can reach more than 10 g/L. The molar conversion rate of ferulic acid is more than 50% and the purity of vanillin is from 80% to 95%. The advantage of this invention includes: repeated use of biocatalyst cells, mild biotransformation condition, low environmental pollution, short production cycle, high product purity and simple purification procedure. It has a great potential for industrial applications.

主权项

1. A method for vanillin production using cells of Amycolatopsis sp. strain (zhp06) or its mutant strains, comprising the steps of:
(a) addition of Amycolatopsis sp. zhp06 cells isolated from a standard cell culture to a substrate solution comprising about 3-40 g/L ferulic acid to form a biotransformation solution, whereby the ferulic acid is converted to vanillin using said Amycolatopsis sp. zhp06 cells as a biocatalyst; and (b) purification of vanillin from said biotransformation solution after 5-12 hours of said conversion reaction of ferulic acid to vanillin, wherein said purification is performed by adding 5-50% (w/v) of macroporous absorbent resin to said biotransformation solution, wherein vanillin is extracted from the solution using said absorbent resin which is then eluted with acetic ether or alcohol at 35-40° C. to produce an eluate solution containing vanillin; and wherein said eluate solution is dried by anhydrous sodium sulfate and further dried by vacuum evaporation to produce a concentrated extract containing 200-230 g/L of vanillin; and subsequently, said concentrated extract is settled at 4° C. for vanillin crystallization thereby purifying vanillin from the biotransformation solution.